How it works: PCR!

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Author’s note: This is part of an installment on how many methods and techniques in biological sciences work, written for educational purposes!  Hopefully, this blog will rise to be something more than silly short stories.

If you’ve ever worked in a biology lab, you’ve probably heard professors, researchers, or even other students talking about PCR.  But what in the world is this strange acronym?
PCR stands for Polymerase Chain Reaction, and is a method for amplifying DNA!  This process can take a few strands of DNA, or even a single strand, and turn it into thousands of (nearly) identical copies! Although it has a few limitations, it is a very rapid, powerful tool, and has many different uses.
So, you may be wondering, how does PCR work?  It’s simple, and all depends on temperature.  But first, a little refresher on DNA.
DNA is made up of two strands, each of which is complementary to the other.  Because they are perfectly complementary, they fit together, bonding perfectly.  Think of it like ripping a piece of paper in half – the two pieces of paper will perfectly match up together, unlike any other ripped piece of paper!  Same thing with DNA.  Once enough complementary bases are in a strand of DNA, it will only fit perfectly with its exact match.
Even though DNA normally sticks together, it does separate – when heated up!  In order to separate the DNA, the solution containing the DNA is heated up to between 95 to 98 degrees Celsius, near-boiling.  At this point, the DNA peels apart into the two different strands, but doesn’t fall apart quite yet.
Now, the enzyme that copies DNA is known as polymerase, and it’s a big, complex protein.  Polymerase attaches on to an exposed single DNA strand, and builds the complementary strand of the DNA.  Bam, DNA copied!  Unfortunately, it’s not quite that easy – there are a couple requirements of polymerase, however, that make it a little tricky to work with.  
The first issue, which made PCR impossible for a long time, is the fact that polymerase is heat-sensitive.  If you heat the protein up too much, it acts a bit like a spaghetti noodle – it falls apart, and no longer performs its DNA-copying function.  Even if you cool it back down, it is still broken.  Now, when you have to heat up the DNA to separate the strands, this becomes an issue.  
The way to solve this problem is actually rather ingenious, in terms of biology.  In some hot vents, such as those found in hot springs in Iceland or at the bottom of the ocean, certain bacteria (known as thermophilic bacteria) are able to thrive.  In order to survive in the boiling-hot water, they have evolved special forms of enzymes that remain stable at very high temperatures.  The polymerase used in PCR comes from one of these bacteria, T. aquaticus, and is thus known as Taq polymerase.  It doesn’t break down in hot water!
The other issue with polymerase, however, is that it can’t start on just a single strand – it needs to start on a double strand.  This means that if you chop a bit out of a double-stranded piece of DNA, the polymerase can copy that area missing its complementary strand.  If you have a single strand by itself, however, there’s no place for the polymerase to attach.
Now, in order to get around this problem, two different short pieces of DNA, known as primers, are added to the mix for PCR.  These primers are usually 8-10 bases each in length, and are specially selected to perfectly fit the DNA strands at either end.  This means that the primer forms a very short section of double-stranded DNA, allowing the polymerase to attach!
The last thing needed for a PCR to work successfully is the raw ingredient – the bases that DNA is built from!  These are easily synthesized, and are added to the mixture so that the polymerase has raw materials to use to build its strands.
So, PCR goes through three steps – denaturing (where the DNA separates into single strands), annealing (where the DNA is cooled off enough for the primers to bond to either end of the single DNA strands), and elongation (where the polymerase attaches to the DNA at the double-stranded primer, and then builds the second strand down the rest of the length of the strand).  Each of these steps is performed at a different temperature, so a PCR machine, also called a thermocycler, rotates a sample between the three temperatures.  Although temperature can vary, denaturing is usually around 96 degrees, annealing happens around 58 degrees, and elongation is generally at 72 degrees.  The thermocycler simply keeps on cycling the temperature of the sample between the three programmed temperatures.
Because the primers/Taq polymerase combination makes a copy of every single-stranded DNA molecule, each cycle should, in theory, double the amount of copied DNA!  Here’s a quick example:
Start with 1 strand.
After 1 cycle, you have 2 strands.
After 2 cycles, you have 4 strands.
After 3 cycles, you have 8 strands.
After 4 cycles, you have 16 strands.
After 5 cycles, you have 32 strands.
As you can see, the amount of DNA grows very rapidly!  Of course, this growth stops if you run out of primer pieces of DNA or raw bases, but in general, this method allows for millions of copies of DNA to be synthesized in a half hour or less.
PCR has a couple other limitations.  Because polymerases don’t copy DNA instantly, there is a limit on how long the copied DNA strands can be.  The maximum length of DNA that can be copied by PCR is about 10,000 base pairs, although some methods can go up to 40,000 base pairs.  PCR also has occasional errors, as the Taq polymerase has an error rate of about 1 in 10,000 bases.  Sometimes, the copied DNA strands aren’t perfect – and that, of course, means that future strands copied from those are also flawed.  
Despite this, however, PCR is a very powerful tool, and is used all the time in biology labs!  And now, you know how it works!

Horror Short 3: "On Repeat"

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As I cruised past the “Mile 66” sign on the road, the metal briefly, brightly illuminated in the haze of falling snow by the glow from my headlights, I fumed silently, my hands gripping the wheel.

Who did that whore think that she was?  How dare she throw me out of her house, as if I was just another bit of garbage?  She may be going through her midlife crisis, I thought furiously to myself, but that gave her no right to get rid of me, her long-suffering husband.

Sure, I had given her a bit of hassle, over the years.  Even I had to admit that we weren’t always the perfect couple, sometimes getting into fights.  And back in my younger days, when I was still a heavy drinker, I may have even gotten physical once or twice.  But never anything serious – I’d never inflicted anything worse than a bruise.  And since I’d sworn off the hard stuff, I had gotten much better.

My headlights caught another sign, this one flashing by the side of the car before I could read it.  It was just another momentarily bright blur in the darkness, like me.  I knew that I still had a few more miles of driving before I reached the turn-off for the cheap motel that was my current destination.  Even in my current state, I was still plenty capable of operating my car.  And I was used to these roads – their ice didn’t bother me, and I could drive through the heavy, silently falling snow.

And, damn it, it was my car!  Just like it was my house, my life, that I had worked hard to build.  And Sara, that insufferable woman, had fit into the plan at first.  She had been young, blonde, pretty, the perfect lady to hang off my arm at parties, to dazzle my bosses with her smile and wit, to rub against my body in the bedroom.

At first, it had all seemed to work out.  She had been everything that I had wanted, had hoped for, and more.  But maybe it had been too perfect.  She had been smart, too smart.  She took away my liquor, stood up to me when I confronted her about it, didn’t back down like a woman was supposed to.  I’d had to hit her.  There hadn’t been any other choice.

Another sign appeared.  This time, I was able to have my eyes up, to read it.  “Mile 66,” it read.  I blinked a couple times.  Hadn’t I passed that one before?  I tried to remember, but my head was fuzzy.  I shrugged and kept on going forward.  Mile 66 meant that I wasn’t there yet.

Tonight . . . tonight, things had boiled over.  All I had wanted was a couple of beers, after work, but that damn woman had come in.  I had tried to cop a feel, show her that I was in the mood, but not only did she shoot me down, but she started yelling at me, not relenting.  All sorts of crap.  I wasn’t what she had married, she wanted a real life, I didn’t care about her – all of it crap.

The next road sign, approaching.  This time, I made sure to slow down, braking cautiously on the snowy road.  “Mile 66,” the sign read.  But this time, I knew that I had passed this sign, had driven more than a mile!

I looked down at the odometer.  Last time I’d checked the car, it had been sprouting eighty-something thousand miles.  I didn’t remember the exact number.  But now, it had almost all been rolled back to zeros.  “2”, the counter read.

A tremor of fear, a strange and barely recognized feeling, suddenly wormed its way in through the anger.  Steering back into the road, I floored the accelerator, shooting down the middle of the street.  It didn’t take long, maybe ten minutes, until another sign was visible behind the wall of falling snow.  I made sure to slow down carefully, staring out at it.  “Mile 66,” I read off.

I looked back down at the odometer.  “3”…

Horror Short 2: "Cut Off The Head"

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The man staggered forward, both hands pressed against his side through the bulky overcoat. The wound was still leaching blood, and he knew that it wouldn’t close. Not until after he had turned, at least.

As he hobbled past the makeshift barricades, he could see eyes, innumerable pairs glowing in the twilight. They didn’t approach, though. They could smell his injury, feel the heat radiating from his clammy skin. They knew that he was infected, would soon join their ranks.

The man lifted his head, blinking as he tried to focus. He knew that the Engine lay ahead. He had worked with the others, the shattered remnants of an obsolete race, figuring out its location. The Contagion Engines, downfall of humanity!  Creators and controllers of the virion plague!

In the dim light, now, the man could see the outline of the massive Engine, complex networks of pipes feeding into the giant, hulking Genesis Sphere. Exhaust vents, aimed upwards, spewed its toxic spores into the sky, a cloud of plague. Gritting his teeth, the man hobbled closer.

Now, in the shadow of the Engine, he saw movement emerging from the darkness. The Guardians. Hundreds of insectile eyes gazed down towards him as the Guardians chittered to their next meal.

The man had known that the Engine would not be undefended. He drew his pistol with a shaking hand, pointing it towards the Guardians.

They buzzed back at him, still slinking forward. They knew that the pistol wouldn’t penetrate their overlapping chitin plates. One, more daring than its fellows, moved forward as it unsheathed its pincers.

The man’s aim was unsteady, but he pulled the trigger, and felt a small twinge of satisfaction as the Guardian erupted into flame. The armor-piercing incendiary shell had burned through the chitin, roasting the abomination from the inside. At least his one shot hadn’t been wasted.

Leaning forward, the man forced himself into a run, past the uncertain Guardians towards the Engine. His other hand dipped into a pocket and his jacket blew open, revealing the heavy vest that covered his wound. The cloth was sodden with blood, but the electronics were sealed and insulated. The man’s hand emerged from the pocket, clutching the detonator.

Standing before the Genesis Sphere, the man turned to face the Guardians, struggling to draw breath as they moved forward to defend the Engine. For one last second, he spread his lips, revealing a toothy, rictus grin. He didn’t know if the creatures could understand him, and he didn’t care.

“Cut off the head,” the man rasped, and slammed down his thumb on the detonator.

Horror Short 1: "Deathtouch"

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“No, please!” I begged her, still cowering in the debris of what had once been my laboratory. “Don’t come any closer!”

As always, she ignored my warning, crouching down in front of me. “Adam, it’s me!  What happened?  Are you all right?”

I shook my head fervently. “No, no!” I cried, trying to retreat further. I was against the wall, however, and she stretched out her hand towards me as I flinched.

For just a moment, I felt the touch of her fingers against my hand, soft and calming. I saw her mouth begin to open for an instant, but no words came out.  Perhaps she could already feel herself diffusing.

And then, before my eyes, she broke apart, vanishing into puffs of smoke and fine particles. Just like everyone else I touched.

I stared around at the empty, ruined lab, that had seemed so full of promise just this morning. The field had collapsed, and I was the last one left. The rest were gone forever, unraveled out of existence.

I lifted my hands, holding them, trembling, before my eyes. Death, death to anything I touched.

Very carefully, no longer holding back my tears, I pressed both of my hands against my head. I closed my eyes as the rush of heat engulfed me.

Brainstorming – Princesses

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Author’s note: This is a brainstorming post.  It will make no clear sense, most likely.  But I need somewhere to write my ideas down and get them in order!

Meridia is a normal person, she likes to believe.  She’s off at college, taking classes, casting dreamy eyes towards the princes who are practicing fencing on the green during the morning between scurrying from building to building.

Now, she knows that most people are destined to remain normal, to end up marrying a baker, a miller, a farmer, or an investment banker.  You know, normal lives.  However, for a few lucky girls, those who have the perfect combination of luck, talent, and work ethic, they transform – into princesses!  These girls, perfect in every way, grow up to marry the princes, taking their pick of the cream of the crop of men and living a charmed life.

Obviously, everyone wants to be a princess.

No one’s quite sure why some young adults, seemingly no different at childhood from everyone else, become these paragons of beauty and grace.  There are plenty of geneticists, psychologists, and anthropologists studying the princesses, searching for a root cause, something for which rich parents could pay lots of money in exchange for giving their child a charmed life.  Unfortunately, no one seems to be making much headway.

Now, princesses seem to have a few common, natural abilities that come with the title.  They can:

  • Communicate with small, often-furry woodland creatures.  This includes, but is not limited to, mice, chipmunks, squirrels, songbirds, skunks, foxes, rabbits, little fish, and so forth.  The animals seem to happily obey orders, although it often helps if the princess is singing…
  • Summon up dresses, ball gowns, crowns, and other princess-like accompaniments (shoes.  Lots of shoes).  No one’s quite sure where they come from.  There’s actually a decent aftermarket for items worn by princesses, and many princesses are able to make a decent second income by selling their items online.
  • Sing.  Sing with perfect, golden, dulcet tones.  They are absolutely loved by choirs.
  • Stay in relatively good shape, thin and limber, no matter how much they eat or exercise.  Something about them just makes them abnormally healthy, apparently.
  • Encourage living things to grow.  Flowers bloom with their kisses, grass sprouts up under foot.  Makes them great for gardening, horrible for clean room environments.  This only seems to work on plants, however; no healing wounds with it.
  • Have persistently good luck.  Somehow, things always seem to turn out well for them.  Several casinos in Vegas have even considered banning them, but they do bring in a lot of onlookers and admirers (who can also break into spontaneous dance numbers).
Clearly, princesses lead a fairly charmed life.  
As for Meridia, aside from some pleasant daydreams, she never really thought of being a princess.  Not that it wouldn’t be welcome, but she just never believed it would happen to her.  However, on the night before her midterms, a raven sitting on her window begins chatting with her.  Is Meridia becoming a princess?  
Or is something else happening?  All of Meridia’s windowsill plants keep dying.  Most of her clothes seem to have turned themselves into a variety of blacks.  And that annoying raven keeps on following her around, offering his sarcastic comments on everything she does.  
On one hand, a princess is a princess, and Meridia has always had her eye on Dulced, one of the top princes.  But will he go for this strange, new, offbeat princess?  Or is Meridia, despite her new changes, still going to be out of luck?

Wasted Potential

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What am I scared of?  That’s an interesting question.

Yes, I know what the question means!  I heard Suzy, here, on my left, say “spiders” already.  I just need to think a moment.  Lemme take a sip of my beer.

Okay, got it.  The thing that scares me most in the world?  Wasted potential.

What do you mean, “What do you mean”?  Do I need to explain it?

Ah.  Guess that I do.  I’ve got a good story here.

So this happened a couple years ago, back when I was still in my undergrad.  Now, if there’s one thing that I’ve learned, it’s to not do your working or studying in your room!  Get distracted in minutes, guarantee you.  Instead, I always like to try and find a coffee shop, someplace with people around so that I can’t just start surfing the web or blaring music.  Makes me keep on reading my textbooks.

So I go into this coffee shop, get my coffee, and I’m looking around.  Place is packed.  I eventually spot the only seat left, and it’s at a table where there’s already another employee, probably on his break or something, just lounging.  I head over, ask if he minds if I sit, no, please, go ahead, thank you, all of that little small talk, great.  I take a seat.

I’m pulling out my books and papers, dumping them onto the table as I’m digging through the crap in my backpack, and the other guy starts looking over at me and my work.  Now, before I go any further, lemme describe this guy.  It’s kinda important.

The guy sitting across from me was definitely in middle age, if not pushing the boundaries of it.  Thinning gray hair, wrinkles around his eyes and on his hands, little bit of a paunch behind that green apron all the employees have to wear, you know.  Typical middle-aged guy, maybe a little run down by life.  Possibly even run over once or twice by life.  But hey, I’m not one to judge.

The guy asks me what I’m studying, I tell him genetics, he makes the appropriate oohs and aahs.  You know.  Everyone gives me that sort of thing, like it’s any tougher to be an expert in genetics than it is to be an expert in medieval literature or something.  But then this guy starts asking questions.  And let me tell ya, he was actually asking good ones!  I’m doing my best to explain the answers, pulling up pictures and diagrams from my books, but this guy got it.

Let me just go back and emphasize this point.  This guy got it.  And this was his first time, too.  Not, “oh, I took this in college but am just now remembering the details of it,” nothing like that.  He knew crap about genetics, but was able to understand everything I said, and make it all fit together logically.  In his head!  After five minutes I was skeptical, after ten minutes I was incredulous, and after fifteen minutes I was amazed.  I was sitting across from a guy that probably had an IQ in the MENSA range.

But then, I start asking him about his life.  “What’s your story?” – you know the drill.  And man, this guy had a story.  But I’ll warn you right now, it’s not a good one.  Oh no, definitely not.

Hold on, I need another beer.

Aah, that’s better.  Keeps the throat wet, you know?

Where was I?  Oh yeah, this guy’s story.  And he had quite a story.  Went off to college, and got a girl pregnant in his freshman year.  Yeah, not even six months in and he’d already knocked up this girl.  Not even a long-term girlfriend, some girl at a party.  He dropped out, tried to help support her, but things sort of fell apart, and they never even got married.  So he doesn’t have alimony, but he’s barely a part of this kid’s life, either.  Sends him money, sees him every couple of months or so.

So now that he’s out of college, well, this guy had to head into the workforce.  And he worked a lot of low pay, hourly jobs.  He eventually managed to pick up an associate’s degree, taking classes at night, and landed himself a decent financial position, but then the recession hit, and back on the street he went.  Back to the hourly wage jobs.  And now, he’s closing in on fifty, and still doing the same thing.  No retirement, no plan for the future.

Now, if you’re not feeling pretty low for this guy, well, I don’t know what’s wrong with ya.  I can tell you, I was feeling it.  So then, before he got up to leave, I had to ask him that one question.

This is the question that, like an iceberg, has a lot more below the surface.  You all probably hear it every single day, in some form.  And you probably all shrug it off with a word or two, a quick little answer that just barely touches the iceberg’s tip.  But it goes a lot deeper than that.  This is the sort of question that can run a whole night’s discussion, just on it’s own.  You all know it.

You don’t know it?  Well, here it is.

“Are you happy?” I asked him.

And oh, ladies and gentlemen, this was the killer blow, the knockout punch.  Because if you’ve been following along, you know that this guy has wasted a hell of a lot of potential.  I knew, from fifteen minutes of talking with him, that he was brilliant.  Not even just smart.  Freaking brilliant.  And he had gone and tossed it all away, squandered a life on meaningless work that really didn’t live up to what he could have done.

But when I asked him that question, I saw him open his mouth.  Saw him start to give that reflex answer, of course, oh yeah, can’t complain, not perfect but a decent life, all of that.  But then he stopped, and I saw that sorrow in his face.  Saw him stop, risking his true self to a stranger.

“No,” he said.

And that one word, right there?  That sent a chill all the way down my spine.  That was, bar nothing, one of the scariest conversations I can remember.  And that, that, is why I’m always questioning my life.  Always taking a step back, peering over my own shoulder to see how I’m doing.  Because I don’t want to destroy my life like that.  I don’t want to find out, down the road, that I have what I fear most:

Wasted potential.

I need another drink.

The Angel on the Train, Part II

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Continued from Part I, here.

“Angel?” the punk repeated slowly, turning to join the rest of us in staring at the young man in the polo.

We all watched, confused, as the young man’s shoulders slumped.  He bent down to one side, fiddling with a shoulder bag, and then withdrew a brightly glowing circle.  At first, I guessed that it had to be some sort of glow stick, joined to make a ring, but it was far too bright.

The young man raised the glowing ring up above his head, holding it in the air about three inches above his short, bed-head styled hair.  He released the ring, and the other members of the train car let out a soft but still audible gasp as it hung in the air, bobbing slightly as the young man below it shifted.

Halo affixed, he then moved forward, taking a seat dejectedly on the steps going up to the car’s slightly higher seats.  “Yeah, angel,” he said hopelessly.  “Guardian angel, for what it’s worth.  Not much.  Man, a couple months ago I was still in the reaping division!”

The rest of us exchanged glances.  Finally, since no one else seemed to be willing to make the move, I stepped up to the plate.  “So, angels are real?” I asked.  “God, the devil, all of that stuff?  It really exists?”

The angel glanced up at me.  “Of course!” he replied.  “Well, I mean, as far as I know.”

“You don’t actually know?”

He shook his head.  “Nah.  It’s like in that one movie, with Matt Damon, right?”

“The Bourne Identity?”  the punk offered.

The angel started to nod, but then paused.  “Maybe?  In any case, I haven’t ever met God or Lucifer myself.  Way too many layers of bureaucracy in the way.  But I’ve heard from folks who say that they know other folks who have seen them, so I’m pretty sure that they exist.”

After this little speech, the angel seemed to lapse off into dejected silence.  The old woman sitting in the handicapped seating area leaned forward and poked him with her cane.  “What happens to us after we die?” she demanded.

The angel looked up as she continued to jab him in the arm with her cane.  “Hey, not my department,” he shot back.  “Besides, you’ve got nearly half a year till you have to worry about it, so calm down.”

After a moment to think through the meaning of this, the old woman squawked wordlessly, and leapt up off her seat.  She scurried to the opposite end of the train car, from where she focused on shooting the evil eye at the angel.  He appeared not to notice, once again drooping his head down between his shoulders.  His halo seemed to dim sadly.

Unexpectedly, I felt a surge of pity.  I got up out of my seat, slowly moving closer to the angel until I could pat him sympathetically on the shoulder.  “There, there,” I murmured, not quite sure what to say.  “Things will work out.”

More hands joined mine on the angel’s shoulders and back.  I glanced up, to find that the other passengers of the train had also come forward to provide comfort.  “Listen,” wheezed the older man who had been sitting next to me.  “Just when things get dark, that’s when you have to pull up hardest, because something better’s about to come along.”

The middle-aged woman nodded.  “He’s right,” she agreed.  “You’re still young, you’ve got your health, and you’ve got a job.  Plenty of time to figure things out.”

The punk kid, standing behind the angel, gave him a soft punch in the shoulder.  “Look, screw my date,” he said.  “She was kind of a jerk anyway.”

As we continued to provide soft words of encouragement, the angel shakily made his way back up to his feet.  Once he was standing, we all felt a jerk beneath us, as the train started rolling again.  In less than a minute, we were pulling into the next station.

The guardian angel looked around at all of us.  “Listen, thank you,” he said, his voice earnest but sounding as if it was about to break.  “You’re all wonderful people, and this is why my job isn’t quite as bad as it could be.”

He headed for the doors, but paused, glancing back at the old woman, who was still glaring at him.  “Listen, I didn’t mean that ‘six months’ thing!” he yelled.  “That was a little human of me, that’s all!”

And with that, he was gone.  To be honest, I still haven’t quite figured out what to make of the whole event.  I was a few minutes late to work, but no one noticed.  And I was certainly nicer to people for the rest of the day.  A couple choice curse words aren’t worth going to Hell, you know?

The Angel on the Train, Part I

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Listen.  You ever have one of those, how can I call it, “spontaneous conversations”?  Know what I mean?  When you’re sitting with a group of strangers, nobody knows each other, and then by the end of a half hour you’re all chatting like you’ve been mates for years.  They don’t happen often, but when they do, it’s great.  Even if you all never see each other again, it’s a little glimpse into someone else’s life.

Anyway, I had one of those today.  And man, was this one a doozie.

I had gotten onto the subway, off on my usual forty-minute commute downtown.  It’s a Thursday, which I personally consider to be the worst day of the week.  It’s day four, the week is dragging along, but you still don’t quite yet have the weekend to look forward to yet.  Everyone’s grumpy and short-tempered on Thursdays.

I got onto the train with no problems, taking a seat between some young punk, dressed in a leather jacket covered in patches and sporting purple and black spiked hair, and an older man with a thin shock of white hair behind his ears.  Both of them begrudgingly made room for me, just one more middle-aged guy in a business suit, headed off to a long day’s work in a stuffy office somewhere.

About fifteen minutes later, however, stuck halfway between a couple of stations, the train began to unexpectedly slow down.  We all glanced up from our distractions.  I had been leafing through a couple financial documents without much interest, but now looked up, as did the other passengers.  I took this chance to glance around.

For a weekday morning, my compartment was surprisingly empty.  Aside from the young punk and the old man, there was also a brown-haired woman who looked to be about my age, similarly dressed in a pantsuit.  A young man, probably in his mid to late twenties, was lounging across a seat, dressed in slacks and a polo.  A very old woman was sitting uncomfortably in the handicapped seats, a cane leaning against the plastic next to her.  As the car slowed down, coming to a stop in the darkness, they all looked up, unsure of what was happening.

A moment later, we heard the metallic rattle of the PA system.  “Sorry, folks, but it looks like there’s a bit of traffic up on the station ahead,” came an indeterminate male voice, not sounding very sorry at all.  “We’ll be sitting here a few minutes.”  Click.  The voice ended without offering any further details.

“Great.  Now I’m going to be late.  Just frickin’ great.”  To my surprise, this loud complaint was voiced sourly by the old woman, still rocking slightly on the bench.

“You?  Last week, my boss caught me sneaking in late.”  The woman who looked about my age was speaking.  “If he catches me late again, I’m going to be fired.  Fired!  Can you imagine finding another job?  In this economy?”  Her voice was rising, beginning to approach panic.  The young man in the slacks and polo leaned forward and gave her an uncomfortable pat on the shoulder.

“So what?  I’m gonna be late for my date!” shot back the punk kid with the brightly colored hair.  He caught me rolling my eyes, and withdrew slightly.  “Matters to me,” he muttered to himself.

“Hey, I’m in just the same situation,” I pointed out, trying to keep my tone reasonable.  “The only thing worse than having to work is being late for work, I’m telling you.”

The older man next to me snorted.  “I’m supposed to be going to see my daughter-in-law for her birthday,” he informed the cabin loudly.  “And she’s going to rip my head off.  She’s never liked me.”

“At least she’ll still be there!” the middle-aged woman yelled.  “I might not have a job to come back to!”

“I’m late for a hospital appointment!” the old woman screeched.

“Screw that!  I’ve already been demoted down to guardian angel, and if I don’t get to my assigned human, I’m going to be busted all the way down to freaking cherub!”

We all turned.  The young man in the slacks and polo was standing up.  As we all stared at him, open-mouthed, he slapped his hands over his mouth, his eyes going wide.

“Angel?” the punk repeated slowly…

Continued here.

Confused Frustration

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Author’s note: this was written on September 5th, so information may be slightly out of date.  I’m probably still feeling pretty much the same, however, since this is being published a good 6 days before even the first Week of Welcome activity.  Eh.

So, for anyone who doesn’t read the author bio on this blog, I’m currently a graduate student.

Oh, wait, what’s that?  My author bio doesn’t say anything about that?  Well, crap.  You’re right.  And just to prove how much of a liar I am, I’m not even a graduate student.  Good lord, I just keep digging myself deeper into this pit of lies.

Let me make an amendment.  I’m not a graduate student YET.  Not for another 11 days, when my program starts.

And I haven’t felt this lost in a while.

Ah, you ask, now hanging on my every word (and probably searching for more opportunities to point out my lies), but why are you lost?  The program hasn’t even started yet!  And you mentioned something about a Welcome Week up there in the italics at the top of this post, so they probably tell you everything you need to know and more!

Yes, oh unseen and mostly imaginary audience, this may be true.  And I hope that it’s true, as it would mean that many of my fears are unfounded and can vanish back into my closet.  But right now, I know next to nothing, and this makes me fear that I’m forgetting about everything.

Case number one: finances.  Now, according to some lovely letters on fancy official school stationary, I have been given a stipend of $26k per year.  Woo!  That’s more money than I made at my previous job, by lots!  I should be able to afford to cover my bed in money and then roll around in it, without the indignity of finding coins lodged in awkward crevices later!

Now, however, I’m not so sure.  From what I seem to be seeing online, that money is given to me before tuition.  And I have to pay tuition.  Not only do I have to pay tuition, but tuition seems to be somewhere between $20,000 and $48,000 per year.

Wait a minute!  How can I be getting 26k but paying out 20k or greater?  Given that my rent for the year is 500/month (which appears to be one of the cheaper options in my city of college residence), this means that, even in the best case scenario, I barely have enough to pay for my apartment.  And I cannot eat my apartment.

(Other issue: apparently I don’t get my financial reimbursements until November.  Kind of a long time to wait, don’t you think, university?  Don’t you??)

Even if money was my only concern at the moment, that wouldn’t be too bad.  Talk to financial office, eat cheap foods, consider second job, consider selling organs, etc.  Plenty of solutions.  But even that’s not my top problem (although it’s a close second).

No, as a graduate student, my number one priority is finding a lab to do research with.  A lab that preferably A) is aligned with my interests, B) that has funding/space for me, and C) that has a professor I get along with.  Mostly in that order.  Now, I have a decent idea of what I want to research.  But finding a lab that seems to match up with this is already proving to be a nightmare, and this is before I’ve even tried contacting them to see if they have funding or space.

AARGH.

On one hand, I’ve been reading online that it’s best to try and set up rotations before school starts.  On the other hand, there are also plenty of people who did not set up their rotations before school starts.  Now, I’m hearing about them on sites where they are lamenting the fact that they didn’t do this earlier, but they’re still around, right?  They made it, didn’t they?  It can’t be that bad.

I’d talk to my graduate mentors about this, but they are out of town for the next week.  I’d talk to my assigned professor about this, but I don’t have one yet.  Internet audience, can you see why I’m stuck?

Right now, I’m just going to try and read as much as I can about graduate school, read up on professors, and hope that some of this gets clearer.  Because, sadly, there isn’t much that I can do.

And that drives me crazy.

/rant

Rumble Strips, Part IV

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The Finale!

Link to Part III.

Close to four months later, I was walking into a restaurant, gazing around the room for the sight of Alex’s unruly hair.  We had decided to get together and catch up on recent events in each others’ lives, and I knew that he had arrived first, as always, at the location we had selected.

I was especially looking forward to this get-together so that I could thank him for his gift.  The watch had buzzed several more times over the last few months, and each time it had ended up saving me from various problems.  One time, it had forced me to pull over to the side of the road, just in time to see a semi truck run through the red light in front of me.  Another time, it had prevented me from eating a “home-made” dish that left my flatmate puking for two days.  Once, it had even prevented me from leaving a friend I had been chatting with.  My insistence on staying forced him to reveal that he was in a deep depression and had been considering taking his own life.  I managed to convince him to talk to his doctor instead.

In short, the watch had proved its value many times over.  It was, quite literally, the best present I had ever received, and I wanted to make sure that Alex was properly thanked.  I also was curious about where he had acquired it; the watch didn’t seem to have any brand markings or logos that I could identify, and my searches on the internet for similar devices had found nothing.

Spotting the shock of mussed hair sitting at one of the tables, I made my way through the restaurant to Alex.  “Hey, sorry I’m late,” I said as I sank into the seat across from him.

“No problem,” he grinned, passing over a menu.  “I’m just glad that we were able to find this time to get together.”

After a brief pause while we both inspected the menus, we placed our orders and resumed talking.  Alex filled me in on his recent developments – he had earned another raise at work, and he elaborated on several pranks that he had gotten away with.  He was always so creative with his revenge stories, and by the end, I was in stitches.

“So, how’s the watch working out?” he asked, after the laughter had subsided.

I smiled back at him.  “I don’t know where you found this thing, but it’s amazing,” I told him.  “It somehow knows about bad things before they happen, and warns me!  It’s saved me from so many bad things, you wouldn’t even believe it.  But I haven’t found anything else like it!  Where did you get it?”

Alex, surprisingly, looked uncomfortable at the question.  “Um, it was actually a gift to me,” he said sheepishly.

“A gift?  From whom?”

“From my dad,” he continued.  “I asked him for a good present idea for a girl, because I know that most of my other ideas from other years haven’t worked out so well.”  I nodded in agreement at this, picturing the robot floor cleaner still sulking in one corner of my apartment.  “And he said that I should get you a watch, and that he had an old one sitting around from someplace.  So I gave it to you.”

I ran my hand over the watch.  This still didn’t tell me much about where it was from, but I had met Alex’s dad before, and knew that he was a kook who accumulated all sorts of odd things.  “Really broke the bank on this present then, huh?” I joked.

This broke the tension, and he narrowed his eyes back at me in a mock glare.  “Hey, you said it’s the best one yet!” he shot back.  But then, his expression grew serious once again.  “Although, this does sort of lead to something that I’ve wanted to talk to you about for a long time.”

I braced myself.  I had a fairly good idea of what was going to come next, and I wasn’t sure that I was ready for it to happen.  Alex, however, was still talking.  “Look, Sue, I’ve known you for a long time,” he started.

“Alex,” I tried to break in, but he held up a hand to forestall me.

“I like you,” he said, the words pouring out in a rush.  “I like you as more than just a friend.  I’ve felt so for ages, but I’ve never been able to tell you, because I was scared of how you’d react.  But now, I’d rather at least say it than continue not knowing whether anything could happen between us or not.”  As he finished this, he stared at me, clearly scared but waiting for an answer.

I stared at him, my mouth possibly hanging open, not sure how to respond.  Of course I had known that he liked me – I had known it so long that it had all but faded from my memory, merely one more bit of background information like the color of his hair or the way that he would rub his ear whenever he was upset and searching for the right words to express his anger.  But I had never planned for how I would respond when he told me, when he changed our relationship from implied to actual.

Finally, my brain kicked into gear, my fight-or-flight response seizing control of my upper functions.  “I’m sorry,” I stammered, jumping to my feet, “but I have to go.” Ignoring Alex’s voice behind me, I hurried towards the door of the restaurant, stretching out my hand for the handle, already finding it hard to see through a veil of tears.

Buzz.  I looked down at my wrist; I could feel, rather than see, my watch going off.  I reached down, hit a couple buttons in an effort to make the buzzing stop, and then reached for the door handle once more.  If anything, the vibrations doubled in intensity, the watch shaking until I could feel my whole arm being shifted back and forth.

I stopped, stunned.  What was the watch trying to tell me?  Lost and confused, I turned around.  Alex was standing there, a few steps away, frozen as my eyes locked on to him.  “Sue,” he said softly.

I took a step back.  The watch buzzed angrily.  I took a step forward.  The buzzing stopped.  Was it really so simple?

Hesitantly, slowly, step by step, I moved forward towards Alex.  He said nothing more, but held out his arms, letting me step forward and into them.  As I felt them curl around me, for one last moment, I held out.  Was this right?  Was I making the right choice?

The watch buzzed once more, a short sharp zing, as though it was able to read my mind and disapproved of what it was seeing.  “Fine,” I said aloud, and turned back to Alex’s smile as he gazed down at me.  “Now what?” I asked him, my words coming out sharper than I had intended.

Alex’s grin grew wider.  “Now this,” he said, and he kissed me.

The watch was silent.  Somehow, I knew that it would be silent for a while.  I was done making bad decisions.